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KMID : 0350519930460020487
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Journal of Catholic Medical College
1993 Volume.46 No. 2 p.487 ~ p.497
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Identification of Serotype of Hantavirus CUMC-88H lsolated from Patients with Hemorrhagic Fever with Renal Syndrome
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Abstract
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Serotype of two CUMC-88H viruses(CUMC-88H1 virus and CUMC-88H2 virus) isolated from acute patients with hemorrhagic fever with real syndrome was identified. Hantaan virus (76/118 strain) and Seoul virus (80-39 strain) were used as standard
reference
viruses.
Propagation of four hantaviruses were compared by the indirect immunoflunrescence antibody (IFA) technique. Infectious viruses in the culture supernatants were calculated by the plaque forming unit. Epitopes of each virus were analysed by the
monoclonal
antibodies. Twelve monoclonal antibodies were applied to differentiate epitopes of four hantaviruses in which 8 monoclonal antibodies were to Hantaan virus strain 76/118 and 4 were to Seoul virus strain B-1 Viral S and M RNA segments were
amplified
using reverse transcriptase directed polymerase chain reaction (PCR). Two oligonucleotide primer pairs specific for S segment of Hantaan virus strain 76/118 and M segment of Seoul virus strain B-1 were used for amplification of genomic sequences
Cleavage patterns of PCR products digested gested with restriction endonuclease Alu I and Hpa I were compared to confirm the identity of the amplified products.
@ES The results were as follow ;
@EN 1. Propagation of CUMC-88H virus in Vero E6 cells was more prolonged than those of Hantaan and Seoul viruses. Biologic property of CUMC-88H virus was maybe different from those of reference viruses.
2. Among 4 hantaviruses, common epitopes(17B1, 18C2, 133E) and unique epitopes (12C2, 13H3, 16E8, 18E7, 19B, B61) were detected by the reactivityes to monoclonal antibodies.
3. By the specific primers, S RNA segments(405-bp) of Hantaan virus and CUMC-88H2 virus were amplified. In contrary, S and M RNA segments(405-bp and 171-pb) of Seoul virus and CUMC-88H1 virus were amplified.
4. With Alu I, the 405-bp amplified products from Hantaan and CUMC-88H2 viruses were cleved into one fragment of 230-bp, and with Hpa I the products into two framgments of 230-bp and 171-bp. With Alu I and Hpa I, the 405-bp amplified products of
Seoul
and CUMC-88H1 viruses cleaved into one fragment of 230-bp, but the 171-bp amplified product. Was not digested by both restriction enzymes.
It suggesta that biologic properties and antigenicity of CUMC-88H virus are different from those of Hantaan and Seoul viruses. According to the molecular characters of viral RNA, the serotype of CUMC-88H virus and CUMC-88H2 virus was identified
as
Seoul
virus and Hantaan virus, respectively.
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KEYWORD
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